Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

John G Doench, Nicolo Fusi, Meagan Sullender, Mudra Hegde, Emma W Vaimberg, Katherine F Donovan, Ian Smith, Zuzana Tothova, Craig Wilen, Robert Orchard, Herbert W Virgin, Jennifer Listgarten, David E Root

Journal: Nature Biotechnology

Publisher: Springer Science and Business Media LLC

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